Research Programme

The HeMiBio research programme is organised in seven workpackages (WPs) five of them being scientific, one dedicated to training & education and one to project management & project coordination.

 

The creation of a liver-bioreactor will be achieved through the following steps:

  • WP1 - Develop tools to engineer three different liver cells (hepatocytes, hepatic stellate cells and hepatic sinusoidal endothelial cells) generated from induced pluripotent stem cells (iPSC) (or expanded using the UpCyte┬« technology) to be used in the hepatic bioreactor
  • WP2 - Incorporate molecular sensors to dynamically measure cell function and toxicity in a high-throughput format
  • WP3 - Develop a 2D-bioreactor for the efficient isolation of differentiated iPSC mixtures by trapping different cell types on micropatterned surfaces
  • WP4 - Generate a 3D liver-simulating device mimicking the human liver, which reproduces the function of the hepatocyte and non-parenchymal liver cells over one month in culture. This will be accomplished by combining the above-mentioned engineered cells and sensors under conditions characterised in the previous step
  • WP5 -
    • Provide proof-of-principle that a liver-simulating device can recreate the toxicity profile in vitro of toxins with a known in vivo toxicity profile over a minimum of one month, including the barrier function of the liver and the effect of inflammatory (and immune) cells in this process
    • Assess the molecular, functional and metabolic phenotype of the hepatocellular, hepatic stellate and hepatic sinusoidal endothelial cell components at all stages of bioreactor development and compare this with that of cells isolated fresh from human livers

 

  

WPS

 

 

 

 

 

 

 

 

 

Schematic representation of tool development and incorporation in diverse bioreactor designs in HeMiBio